Journal: Nucleic Acids Research

Article Title: Ensembl 2005

doi: 10.1093/nar/gki138

Figure Lengend Snippet: Screenshot of Ensembl multicontigview. The view shows genome sequence with annotation from human, mouse and chicken, aligned according to DNA–DNA similarity, shown in green. Pairwise similarity is shown between the ‘primary’ genome (human in this case) and each of the other genomes. Menus allow additional genomes to be added to the display. The ‘P’ button allows a different genome to be selected as the primary one. Genes automatically identified as putative orthologues are linked by blue lines. The region shown is centred around the HOXB3 gene in the HOX cluster on human chromosome 17 and is shown to be syntenic with a region on mouse chromosome 11. All Ensembl known gene structures are conserved and have been correctly identified as orthologues. Two novel Ensembl gene structures predicted in mouse are not seen in human. It would be interesting to investigate the corresponding region in human to understand why they were not predicted there. Features such as alignments to cDNAs and proteins can be turned on using the menus to facilitate such a comparison. Putative orthologue prediction and DNA similarity show a much weaker and incomplete link to a region in the chicken genome; however this is on chromosome Un, which is a fake chromosome composed of fragments that could not be mapped onto chromosomes in the current assembly. Whereas the chicken fragment contains HOXB3 and HOXB1 , HOXB4 and others are absent. The putative chicken orthologue for human HOXB4 is found in another chicken fragment in the fake Un chromosome (data not shown), suggesting that the chicken equivalent of the human chromosome 17 HOX cluster is fragmented in the current chicken assembly.

Article Snippet: The Epigenomics Consortium ( ) ( http://www.epigenome.org/ ) has built a viewer based on Ensembl web drawing code and DAS sources ( http://www.sanger.ac.uk/PostGenomics/epigenome/ ).

Techniques: Sequencing, Comparison

Journal: Nucleic Acids Research

Article Title: Ensembl 2005

doi: 10.1093/nar/gki138

Figure Lengend Snippet: Screenshot of Ensembl genesnpview. This new gene-centric view shows in a single display the genomic context of a gene and its surrounding SNPs. The figure shows the region of the human genome around the HOXB3 gene in the HOX cluster on chromosome 17. The display shows three different resolutions: the genes over a 270 kb region are shown around HOXB3 ; the HOXB3 gene itself (gene id ENSG00000120093) and the HOXB3 transcript (transcript id ENST00000311626) with intragenic sequence and introns truncated so that it is mainly CDS and untranslated region (UTR) sequence that is shown. By default the flanking regions are truncated to 50 bp. This can be changed with the ‘Context’ menu and in this case has been set to 200 bp, revealing six intronic SNPs. It can be seen that the CDS of the transcript includes one known protein domain (the Pfam PF00046 homeobox domain). There are only two SNPs that fall within the CDS and only one of these is non-synonyomous leading to a proline (P) to threonine (T) amino acid change in the second exon as a result of an A to C base change. There is a further flanking SNP (C to T change) in the 3′-UTR. A table immediately below the figure provides more information about the SNPs that intersect the transcript being viewed. The three menu bars ‘SNP class’, ‘Validation’ and ‘SNP type’ allow the SNPs being displayed to be filtered. If there were multiple transcripts for the gene selected, they would each be displayed. The view thus combines data in a single view that can partly be found in contigview, transview, protview and snpview.

Article Snippet: The Epigenomics Consortium ( ) ( http://www.epigenome.org/ ) has built a viewer based on Ensembl web drawing code and DAS sources ( http://www.sanger.ac.uk/PostGenomics/epigenome/ ).

Techniques: Sequencing, Biomarker Discovery